As a PacBio Certified Service Provider, MGI continues to work with the latest technology, a Sequel II System. The Single Molecule, Real-Time (SMRT) Sequencing is the core technology for powering this long-read technology.
The instrument has two sequencing mode options.
- Continuous Long Reads (CLR) Sequencing Mode – CLR data correspond to reads with a subread length approx. equivalent to the polymerase read length indicating that the sequence is generated from a single continuous template from start to finish – CLR Sequencing Mode is useful for applications such as WGS de novo assembly and structural variation detection.
- Consensus Sequencing (CCS) Sequencing Mode – CCS data correspond to reads generated from the consensus sequence resulting from alignment between subreads taken from a single ZMW – CCS Sequencing Mode is useful for applications such as variant detection and Iso-Seq studies.
Run time ranges from 30 minutes to 30 hours per SMRT cell with 1 to 4 SMRT cells per run.
Key applications for the technology include:
- De novo genome assembly
- Structural variation analysis
- Gene annotation
- Targeted analysis
- Whole genome analysis
- RNA isoform sequencing (Iso-Seq)