Salmonella enterica serovar Paratyphi B
Salmonella Paratyphi B
Salmonella (Salmonella enterica) Credit: Photo courtesy of Nature (Permission Pending)
Habitat
The bacterium Salmonella Paratyphi B is host-specialized, for it grows well and causes disease only in humans, whereas most strains of Salmonella can grow in the gut of almost all animals, both domesticated and wild. Humans usually acquire Salmonella Paratyphi B by the ingestion of water or of food that has been contaminated through fecal contact with humans. Most isolates of Salmonella belong to the species S. enterica, which is further subdivided into many serovars based on antigens on their surface; one of these serovars is Paratyphi B. Paratyphi B is quite diverse and human infection is sometimes not associated with human to human system infection but rather associated with foodborne infection (Prager et al, 2003).
Biology
Salmonella is a Gram-negative bacterium, a cylindrical rod of size about 2 microns by 0.5 microns, thus much smaller than the cells of higher organisms such as humans. In culture, the bacterium will grow on artificial media to form colonies. Salmonella Paratyphi B causes enteric fever in humans; the disease closely resembles Typhoid Fever, which is caused by the related serovar Salmonella Typhi. Salmonella Paratyphi B multiplies in the gastrointestinal tract of humans, and then it penetrates the intestinal mucosa and is transferred via the lymph and the blood to deeper tissues such as the liver and spleen where it survives in macrophages. The incubation period is 7 to 14 days, and the patient is often seriously ill with symptoms including fever, diarrhea, and abdominal pain. The disease has a prolonged course of 3 to 4 weeks, unless antibiotic therapy is used.
Sequencing
The specific strain of S. Paratyphi was isolated from the stool of a female in Penang Malaysia, and was obtained from Kwai-Lin Thong. It can be obtained from the Salmonella Genetic Stock Center as SGSC4150 (www.ucalgary.ca/~kesander). The genome was sequenced to 8X coverage, using plasmid and fosmid libraries, and was finished to an error rate of less than 1 per 10,000 bases. Automated annotation was performed and manual annotation continued in the labs of Michael McClelland and Kenneth Sanderson. The sequence is complete and can be accessed in GenBank (CP000886.1). The National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) funded this project.
Contacts
| Name | Affiliation |
|---|---|
| Patrick Minx | The Genome Institute, Washington University School of Medicine |
| Michael McClelland | Cancer Genetics Program, Sidney Kimmel Cancer Center |
| Kenneth Sanderson | Salmonella Genetic Stock Centre, University of Calgary |
Sequences & Maps
Assemblies
| Name | Date | Description | Blast DBs |
| Salmonella_enterica_serovar_Paratyphi_B-1.0 | Feb 02, 2006 | 11.6X | contigs supercontigs |
| Salmonella_enterica_serovar_Paratyphi_B-2.0 | Apr 05, 2006 | 11.7X | contigs supercontigs |
| Salmonella_enterica_serovar_Paratyphi_B-3.0 | May 08, 2006 | 11.7X | contigs supercontigs |
Publications
McClelland M, Florea L, Sanderson K, Clifton SW, Parkhill J, Churcher C, Dougan G, Wilson…
Comparison of the Escherichia coli K-12 genome with sampled genomes of a Klebsiella pneumoniae and three salmonella enterica serovars, Typhimurium, Typhi and Paratyphi.
Nucleic Acids Res. 2000 Dec 15;28(24):4974-86. PubMed | View Abstract
McClelland M, Wilson RK.
Comparison of sample sequences of the Salmonella typhi genome to the sequence of the complete Escherichia coli K-12 genome.
Infect Immun. 1998 Sep;66(9):4305-12 PubMed | View Abstract
